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Nucleic Acids Research, 1986, Vol. 14, No. 11 4471-4481
© 1986

Articles

Sequence organization of the circular plasmid pKDl from the yeast Kluyveromyces drosophilarum

X. J. Chen, M. Saliola+, C. Falcone+, M. M Bianchi and H. Fukuhara*

Institut Curie, Section de Biologic, Bailment 110, Centre Universitaire Orsay 91405, France +Department of Cellular and Developmental Biology, University of Rome Rome 00185, Italy

*To whom correspondence should be addressed

Received March 13, 1986. Revised May 13, 1986. Accepted May 13, 1986.

pKDl is the only circular plasmid known in the genus Kluyveromyces. Nucleotide sequence analysis has revealed that this 4757 base-pairs long plasmid contained three major open reading frames, A, B, and C, and a pair of inverted repeats of 346 base-pairs. The molecule exists in two isomeric forms generated by internal recombination at these repeats. The functional organization of pKDl genome appears to be quite analogous to that of the 2u plasmid of Saccharomyces cerevisiae. There is however little homology of sequences between these plasmids, except that the gene A has a dispersed but significant homology with the FLP recombinase gene of the 2u plasmid. S.cerevisiae cells can be transformed by derivatives of pKDl carrying URA3 gene as a selection marker.


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