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Nucleic Acids Research, 1986, Vol. 14, No. 11 4483-4497
© 1986

Articles

Osteontctin mRNA: distribution in normal and transformed cells

Marian F. Young, Mark E. Bolander*, Agnes A. Day, Camille I. Ramis, Pamela Gehron Robey, Yoshihiko Yamadav* and John D. Termine

Bone Research Branch Bethesda, MD 20892, USA *Bone Research Branch and Laboratory of Developmental Biology and Anomalies, National Institute of Dental Research, National Institutes of Health Bethesda, MD 20892, USA

Received March 6, 1986. Accepted May 7, 1986.

Overlapping cDNA clones encoding bovine osteonectin were isolated from a {gamma}gtll expression library constructed from bovine bone cell mRNA. The longest clone, {gamma}On 17 (insert size 2.0 kb) was studied in detail. The clone was shown to encode osteonectin by hybrid select translation experiments and by DNA sequence analysis. Northern analysis of bone cell RNA showed the length of the osteonectin mRNA to be 2.0 kb. Osteonectin message was found in bone but not in soft tissue (liver and brain) preparations consistent with the distribution of the protein in these tissues. On the other hand, osteonectin message was observed in tendon, a tissue in which little or no osteonectin protein is found in vivo. Hybridization of osteonectin cDNA was detected in cells from a number of species including human, rat, mouse and chick. The level of osteonectin mRNA was drastically decreased in chick embryo fibroblasts transformed by Rous sarcoma virus.


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