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Nucleic Acids Research, 1986, Vol. 14, No. 11 4605-4616
© 1986

Articles

Cloning a selected fragment from a human DNA ‘fingerprint’: isolation of an extremely polymorphic minisatellite

Zilla Wong, Victoria Wilson, Alec J. Jeffreys and Swee Lay Thein+

Department of Genetics, University of Leicester University Road, Leicester LEI 7RH +MRC Molecular Haematology Unit, Nuffield Department of Clinical Medicine, John Radcliffe Hospital Headington, Oxford OX3 9DU, UK

Received April 8, 1986. Accepted May 7, 1986.

A large hypervariable DNA fragment from a human DNA fingerprint was purified by preparative gel electrophoresis and molecular cloning. The cloned fragment contained a 6.3 kb long minisatellite consisting of multiple copies of a 37 bp repeat unit. Each repeat contained an 11 bp copy of the "core" sequences, a putative recombination signal in human DNA. The cloned minisatellite hybridized to a single locus in the human genome. This locus is extremely polymorphic, with at least 77 different alleles containing 14 to 525 repeat units per allele being resolved in a sample of 79 Individuals. All alleles except the shortest are rare and the resulting heterozygosity is very high (97%). Cloned minisatellites should therefore provide a panel of extremely informative locus-specific probes ideal for linkage analysis in man.


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