Nucleic Acids Research, 1986, Vol. 14, No. 11 4617-4624
© 1986
Articles |
Expression in mammalian cells of a gene from Streptomyces alboniger conferring puromycin resistance
Centre de Biologìa Molecular, Universidad Autònoma de Madrid Canto Blanco, 28040 Madrid, Spain
*To whom correspondence should be addressed
Received March 27, 1986. Accepted May 2, 1986.
The gene encoding a puromycin N-acetyl transferase from Streptomyces alboniger has been cloned next to the SV40 early promoter in a mammalian cells-Escherichia coli shutle vector. When this construction was introduced into VERO cells it expressed the relevant enzymic activity. Moreover, the puromycin N-acetyl transferase gene has been used as a dominant marker for the selection of transformed mammalian cells able to grow in the presence of the antibiotic.
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