Nucleic Acids Research, 1986, Vol. 14, No. 12 4923-4937
© 1986
Articles |
Replication of phage 
29 DNA in vitro: role of the viral protein p6 in initiation and elongation
Centro de Biologìa Molecular (CSIC–UAM), Universidad Autònoma Canto Blanco, 28049 Madrid, Spain
Received February 28, 1986. Revised May 23, 1986. Accepted May 23, 1986.
The 
29 protein p6 stimulates the formation of the protein p3–dAHP initiation complex when added to a minimal system containing the terminal protein p3, the 29 DNA polyraerase p2 and 29 DNA–protein p3 complex,by decreasing about 5 fold the Km value for dATP. In addition, protein p6 stimulates elongation of the p3–dAMP initiation complex. Whereas the effect of protein p6 on initiation is similar with protein p3–containing fragments from the right or left 29 DNA ends, the stimulation of elongation is higher with the right than with the left 29 DNA terminal fragment, suggesting DNA sequence specificity. The stimulation by protein p6 of the initiation and elongation steps of 29 DNA replication does not require the presence of the parental protein p3 at the 29 DNA ends. No effect of protein p6 was obtained on the elongation of the template-primer poly(dT)–(dA)12–18 by the 29 DNA polymerase.
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