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Nucleic Acids Research, 1986, Vol. 14, No. 12 4969-4986
© 1986


Articles

Occurrence of 9 homologous repeat units in the external spacer region of a nuclear maize rRNA gene unit

Christian Toloczyki and Günter Feix

Institut für Biologie III der Universität Freiburg, Schänzlestrasse 1, D-7800 Freiburg, FRG

Received December 12, 1985. Accepted May 7, 1986.

A 14 kb maize DNA fragment carrying nuclear rRNA genes and spacer regions was isolated and characterised by restriction enzyme mapping. A complete 3020 bp long external spacer region was sequenced and revealed 9 tandemly arranged 200 bp long repeat units with high homology. The repeat units lie upstream from two prominent S1 mapping signals. The sequence of a typical repeat unit is compared to a corresponding 130 bp long wheat repeat unit. The possible functional relevance of the repeat units is discussed.


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