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Nucleic Acids Research, 1986, Vol. 14, No. 14 5615-5627
© 1986

Articles

Gene activation properties of a mouse DNA sequence isolated by expression selection

Volker von Hoyningen-Huene, Chris Norbury, Moira Griffiths and Mike Fried

Department of Tumour Virus Genetics, Imperial Cancer Research Fund Lincoln's Inn Fields, London WC2A 3PX, UK

Received May 29, 1986. Accepted July 2, 1986.

The MES-1 element was previously isolated from restricted total mouse cellular DNA by "expression selection" - the ability to reactivate expression of a test gene devoid of its 5' enhancer sequences (1). Mes-1 has been tested in long-term transformation and short-term CAT expression assays. In both assays MES-1 is active independent of orientation and at a distance when placed 5' to the test gene. The element is active with heterologous promoters and functions efficiently in both rat and mouse cells. MES-1 activates expression by increasing transcription from the test gene's own start (cap) site. Thus the expression selection technique can be used for the isolation of DNA sequences with enhancer-like properties from total cellular DNA.


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