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Nucleic Acids Research, 1986, Vol. 14, No. 14 5641-5650
© 1986

Articles

Primary structure of a proteinase inhibitor II gene from potato (Solanum tuberosum)

Michael Keil, Jose Sanchez-Serrano, Jeff Schell and Lothar Willmitzer

Max-Planck-Institut fuer Zuechtungsforschung, 5000 Koeln 30, FRG

Received May 21, 1986. Revised July 1, 1986. Accepted July 1, 1986.

The isolation and characterization of a genomic clone encoding proteinase inhibitor II of potato (Solanum tuberoaum is described. The structure of this gene was determined by sequencing a genomic fragment of about 2 kb containing the entire RNA coding as well as about 900 nucleotides of the 5'-upstream and 250 nucleotides of the 3'-downstream region. The transcription start site was determined by RNase protection experiments.

The comparison of the genomic sequence with cDNA sequences reveals the presence of one intron with a length of 117 nucleotides. The genomic clone contains an open reading frame of 462 nucleotides allowing for a protein of 154 amino acids.

The proteinase inhibitor II gene displays typical features of eucaryotic genes. The sequence TATAAA is found 26 nucleotides upstream of the transcription initiation site and the sequence CAAAT at position - 103. In the 3'-region the sequence AATAA is found 33 nucleotides in front of the poly-A addition site.


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