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Nucleic Acids Research, 1986, Vol. 14, No. 14 5741-5760
© 1986

Articles

Complementarity of sequences in low molecular weight RNAs to regions of messenger and ribosomal RNAs

E.S. Maxwell* and T.E. Martin+

Department of Molecular Genetics and Cell Biology, University of Chicago 1103 East 57th Street, Chicago, IL 60637, USA

+To whom correspondence should be addressed

Received March 11, 1986. Revised June 3, 1986. Accepted June 26, 1986.

Total low molecular weight nuclear RNAs of mouse ascites cells have been labeled in vitro and used as probes to search for complementary sequences contained in nuclear or cytoplasmic RNA. From a subset of hybridizing lmw RNAs, two major species of 58,000 and 35,000 mol. wt. have been identified as mouse 5 and 5.8S ribosomal RNA. Mouse 5 and 5.8S rRNA hybridize not only to 18 and 28S rRNA, respectively, but also to nuclear and cytoplasmic poly(A ) RNA. Northern blot analysis and oligo-dT cellulose chromatography have confirmed the Intermolecular base-pairing of these two small rRNA sequences to total poly(A+) RNA as well as to purified rabbit globin mRNA. 5 and 5.8S rRNA also hybridize with positive (coding) but not negative (noncoding) strands of viral RNA. Temperature melting experiments have demonstrated that their hybrid stability with mRNA sequences 1s comparable to that observed for the 5S:18S and 5.8S:28S hybrids. The functional significance of 5 and 5.8S rRNA base-pa1r1ng with mRNAs and larger rRNAs 1s unknown, but these Interactions could play Important coordinating roles in ribosome structure, subunit Interaction, and mRNA binding during translation.

*Present address: Department of Biochemistry, North Carolina State University, Raleigh, NC 27695-7622, U.S.A.


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