Nucleic Acids Research, 1986, Vol. 14, No. 14 5813-5826
© 1986
Articles |
Translationsl regulation of expression of the bacteriophage T4 lysozyme gene
Department of Molecular, Cellular and Developmental Biology, University of Colorado Boulder, CO 80309 1Department of Biochemistry, University of Washington Seattle, WA 98195, USA
Received March 6, 1986. Revised June 24, 1986. Accepted June 24, 1986.
The bacteriophage T4 lysozyme gene is transcribed at early and late times after infection of E. coli, but the early mRNA is not translated. DNA sequence analysis and mapping of the 5' ends of the lysozyme transcripts produced at different times after T4 infection show that the early mRNA is Initiated some distance upstream from the gene. The early mRNA is not translated because of a stable secondary structure which blocks the translational initiation site. The stable RNA structure has been demonstrated by nudease protection in vivo After DNA replication begins, two late promoters are activated; the late transcripts are initiated at sites such that the secondary structure can not form, and translation of the late messages occurs.
*Present address: Synergen Associates, 1885 33rd Street, Boulder, CO 80301, USA
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