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Nucleic Acids Research, 1986, Vol. 14, No. 14 5885-5900
© 1986

Articles

Use of a 1- hydrorybenzotriazole activated pbospborylating reagent towards the synthesis of short RNA fragments in solution

E. de Vroom, A. Fidder, J.E. Marugg, G.A.van der Marel and van J.H. Boom

Gorlaeus Laboratories, Department of Organic Chemistry PO Box 9502, 2300 RA Leiden, The Netherlands

Received May 9, 1986. Revised June 30, 1986. Accepted June 30, 1986.

Evidence will be presented to show that the reagents 3a-c (X=0) obtained by the reaction of 2-chlorophenylphosphorodichloridate with 1- hydroxybenzotri-azole or l-hydroxy-6-trifluoromethyl(6-nitro)benzotriazoles, respectively, do not give, under normal coupling conditions, phosphorylation of the lactam functions in uracil or guanine. Reagent 3b (X=0) proved to be very convenient for the in situ formation of 3'–5'-phosphotriester linkages. The latter will be illustrated by the synthesis of several RNA fragments.


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