Nucleic Acids Research, 1986, Vol. 14, No. 15 6067-6083
© 1986
Articles |
Association of the herpes simplex virus regulatory protein ICP4 with specific nucleotide sequences in DNA
Department of Microbiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
*To whom correspondence should be addressed
Received May 12, 1986. Accepted July 10, 1986.
We report that the herpes simplex virus (HSV) transcription regulatory protein designated ICP4 is a component of a stable complex between protein and specific nucleotide sequences in double-stranded DNA formed by addition of exogeneous DNA to either a crude extract obtained from HSV-1 infected cells or a partially purified preparation of native ICP4. DNA sites which are bound directly or indirectly to ICP4 have been designated ICP4/protein binding sites. Three independent ICP4/protein binding sites have been identified by DNAse footprinting; two are in the vector pBR322 and one is located approximately 100 nucleotides upstream from the HSV glycoprotein D mRNA cap site. Comparison of the nucleotide sequences in these three sites reveals several regions of homology. We propose that the sequence 5'ATCGTCNNNNYCGRC-3' (N = any base; Y = pyrimidine; R = purine) forms an essential component of the ICP4/protein binding site.
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