Nucleic Acids Research, 1986, Vol. 14, No. 15 6085-6099
© 1986
Articles |
The DNase I sensitive domain of the chicken Iysozyme gene spans 24 kb
Institut für Physiologische Chemie, Physikalische Biochemie und Zellbiologie der Universität München Schillerstrasse 44, D-8000 München 2, FRG
*To whom correspondence should be addressed
Received May 1, 1986. Revised July 8, 1986. Accepted July 8, 1986.
We have determined the DNase I sensitive chromatin domain of the lysozyme gene in the hen oviduct. When nuclei were digested with DNase I, about 14 kb of upstream and 6 kb of downstream sequences in addition to the 4 kb long transcribed region were preferentially degraded. The transcription start site is located near the center of the approximately 24 kb long sensitive domain. At the 3' boundary there is a rather abrupt transition from the DNase I sensitive to the resistant chromatin configuration whereas at the 5' border this transition occurs in a gradual fashion over 6–7 kb of DNA. No obvious correlation between the boundaries of the domain and repetitive sequences could be established. DNase I-hypersensitive sites are clustered within the boundaries of the sensitive domain which seems to represent a functional unit of the gene.
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