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Nucleic Acids Research, 1986, Vol. 14, No. 15 6227-6245
© 1986

Articles

Efficient methods for attaching non-radioactive labels to the 5' ends of synthetic oligodeoxyribonucleotides

Sudhir Agrawal, Chris Christodoulou and Michael J. Gait

MRC Laboratory of Molecular Biology Hills Road, Cambridge CB2 2QH, UK

Received June 6, 1986. Accepted July 2, 1986.

The syntheses are described of two types of linker molecule useful for the specific attachment of non-radioactive labels such as biotin and fluorophores to the 5' terminus of synthetic oligodeoxyribonucleotides. The linkers are designed such that they can be coupled to the oligonucleotide as a final step in solid-phase synthesis using commercial DNA synthesis machines. Increased sensitivity of biotin detection was possible using an anti-biotin hybridoma/peroxidase detection system.


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