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Nucleic Acids Research, 1986, Vol. 14, No. 16 6357-6373
© 1986

Articles

The TRP4 gene of Saccharomyces cerevisiae: isolation and structural analysis

Rolf Furter*, Gerhard Paravicini, Markus Aebi, Gerhard Braus, Franziska Prantl, Peter Niederberger and Ralf Hütter

Mikrobiologisches Institut, Eidgenössische Technische Hochschule, ETH-Zentrum CH-8092 Zürich, Switzerland

Received July 3, 1986. Accepted July 29, 1986.

The TRP4 gene of Saccharomyces cerevisiae, encoding the anthranilate phosphoribosyl transferase, was isolated and subcloned by functional complementation in yeast. A 2 kb fragment containing information for a polypeptide of amino acids and the 5';- and 3'-flanking regions was sequenced. The TRP4 transcript was identified and mapped with S1 nuclease. Homologies to two prokaryotic genes encoding the same function, and sequences potentially involved transcription start and termination and in regulation of TRP4 gene expression are discussed.

*Present address: Department of Genetics, University of Washington, Seattle, WA 98195, USA


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