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Nucleic Acids Research, 1986, Vol. 14, No. 16 6375-6392
© 1986

Articles

Intron sequences modulate feather keratin gene transcription in Xenopus oocytes

A.M. Koltunow*, K. Gregg and G.E. Rogers

Department of Biochemistry, University of Adelaide, GPO Box 498, Adelaide, South Australia 5001, Australia

*To whom reprint requests should be sent

Received June 17, 1986. Accepted July 29, 1986.

We have shown that chicken feather keratin genes are transcribed at extremely low levels in Xenopus oocytes. Primer extension reactions using carefully selected primers have shown that none of the detectable transcripts spliced. When the single intron, located in the 5' untranslatedregion, removed from thegene, we observed a 5-fold increase in transcript initiation from the in vivo "cap" site. Significantly, this 5-fold increase also observed when the feather keratin intron was replaced with a similar sized fragment from pBR322. We conclude that the efficiency of accurate transcriptional initiation of chicken feather keratin genesin the oocyte is affected by ONA sequences within the intron.


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