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Nucleic Acids Research, 1986, Vol. 14, No. 16 6551-6564
© 1986

Articles

Sequence of 2,617 nucieotides from the 3' end of Newcastle disease virus genome RNA and the predicted amino acid sequence of viral NP protein

Nobuhiro Ishida, Hideharu Taira1, Toshiko Omata1, Kiyohisa Mizumoto2, Seisuke Hattori, Kentaro Iwasaki1 and Masao Kawakita

Department of Pure and Applied Sciences, College of Arts and Sciences, University of Tokyo Meguro-ku, Tokyo 153 1Department of Physiological Chemistry, Tokyo Metropolitan Institute of Medical Science Bunkyo-ku, Tokyo 113 2Department of Chemistry, Institute of Medical Science, University of Tokyo Minato-ku, Tokyo 108, Japan

Received June 11, 1986. Accepted July 29, 1986.

DNA fragments complementary to the Newcastle disease virus genome (strain D26) were cloned and sequenced. The sequence of 2,617 nucleotides from the 3' end of the genome was determined and an open reading frame (OP–1) consisting of 1,467 nucieotides, most likely encoding NP protein, was found in this region. This was followed by a second unfinished open reading frame (OP–2) of at least 729 nucleotides which continued beyond the 2,617th nucleotide. Another relatively short (312 nucleotides long) open reading frame (OP–2') was found overlapping with OP–2, but its significance is still unclear. The amino acid sequence deduced from the nucleotide sequence of OP–1 showed a moderate homology to that of the NP protein of Sendai virus in the central portion of the peptide. The leader sequence of 53 nucieotides was also identified.

The 5' end of mRNAs synthesized in the infected cells was analyzed and found to be m7 GpppA, suggesting that the transcription of viral mRNAs starts with A, but not with G residue.


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[Abstract] [Full Text] [PDF]


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