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Nucleic Acids Research, 1986, Vol. 14, No. 16 6565-6577
© 1986

Articles

B-lymphocyte targeting of gene expression in transgenic mice with the immunoglobulin heavychain enhancer

P. Gerlinger, M. LeMeur, C. Irrmann, P. Renard, C. Wasylyk and B. Wasylyk

Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologie Moléculaire et de Génie Génétique de l'INSERM, Institut de Chimie Biologique, Faculté de Médecine 11 rue Humann, 67085 Strasbourg Cédex, France

Received June 11, 1986. Accepted July 22, 1986.

A hybrid gene containing rabbit ß -globin structural sequences (–9 to $1650), and a chicken conalbumin gene promoter ($62 to –102) in the place of the ß –globin promoter (upstream from –9), was inactive in 5 different transgenic mouse line. Adding the mouse immunoglobulin heavy-chain (IgH) enhancer to this construction specifically stimulated expression in B-cells. These results show that IgH enhancer is specifically active in B-cells. Expression of the hybrid gene was low compared to the endogenous immunoglobulin heavy and light-chain genes. Substituting the mouse immunoglobulin {kappa} light-chain gene (Ig {kappa}) promoter ($4 to –800) for the heterologous conalbumin promoter was not sufficient to restore gene expression to level of the endogenous genes. In addition to the reproducible B cell expression, we also found inheritable unexpected expression in certain tissues, which varied from line to line.


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[Abstract] [PDF]


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