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Nucleic Acids Research, 1986, Vol. 14, No. 17 7031-7045
© 1986

Articles

Restriction endonucleases from Herpetosiphon giganteus: an example of the evolution of DNA recognition specificity?

Philip R. Whitehead*, David Jacobs and Nigel L. Brown+

Department of Biochemistry, University of Bristol, University Walk Bristol BS8 1TD, UK

+To whom correspondence should be addressed

Received July 2, 1986. Accepted August 6, 1986.

We describe the partial purification and characterisation of five Type II restriction endonucleases from two strains of Herpetosiphon giganteus. One of the activities, HgiJII, was the first enzyme found that cleaves DNA at the family of related sequences 5'-G-R-G-C-Y/C-3'. This enzyme may be related to the enzyme HgiAI from a different strain of the same species, and which cleaves at the sites 5'-G-W-G-C-W/C-3'. We have shown that DNAs from the strains producing HgiAI and HgiJII are resistant to both of these restriction endonucleases.

The remaining four enzymes described here share recognition and cleavage specificities with other restriction endonucleases. The evolution of Type II restriction-modification systems and their role in vivo are discussed.

*Present address: Department of Clinical Immunology, Royal Perth Hospital, Perth, Western Australia 6001.


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