Nucleic Acids Research, 1986, Vol. 14, No. 17 7047-7058
© 1986
Articles |
Nucleotide sequence of the tetM tetracycline resistance determinant of the streptococcal conjugative shuttle transposon Tn1545
Unité des Agents Antibactériens, CNRS UA 271, Institut Pasteur 28, rue du Dr Roux, 75724 Paris Cedex 15, France
*To whom all correspondence should be addressed
Received June 26, 1986. Accepted August 6, 1986.
The nucleotide sequence of the tetracycline resistance gene tetM encoded by streptococcal conjugative shuttle transposon Tn1545 has been determined. The resistance gene was identified as a coding sequence of 1917 base pairs corresponding to a protein with a Mr of 72,500 daltons. This value is in good agreement with that, 68,000 daltons, estimated by SDS-polyacrylamide gel electrophoresis of Escherichia coli minicell extracts. The tetM gene product does not exhibit any sequence homology with either the Gram-negative (tetA, tetB and tetC), or the Bacillus and Staphylococcus tetracycline resistance protien. The average hydropathy value of the tetM gene product (–0.21) contrasts with those calculated for the other TET proteins which are markedly hydrophobic (0.76 to 0.93). Hybridization experiments performed with an intragenic tetM probe do not support the claim [Taylor, D. (1986), J. Bact. 165, 1037–1039)] that tetracycline resistance in Campylobacter is due to acquisition of tetM.
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