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Nucleic Acids Research, 1986, Vol. 14, No. 17 7059-7070
© 1986

Articles

Globin gene transcripts can utilize histone gene 3' end processing signals

E. Whitelaw, A. Coates and N.J. Proudfoot

Sir William Dunn School of Pathology, University of Oxford South Parks Road, Oxford OX1 3RE, UK

Received June 24, 1986. Accepted August 6, 1986.

Deletion of the poly(A) site from the human a globin gene results in a defective gene that produces very little stable mRNA as compared to the intact gene, presumably due to the instability of the mRNA. However, if the a poly(A) site is replaced by mouse histone H4 3' end processing signals, significant levels of hybrid {alpha}/H4 mRNA are obtained and the transcripts formed are cytoplasmic and poly(A)–. When both mouse histone 3' end processing signals and the {alpha} globin poly(A) site signals are placed in tandem after the {alpha} globin gene promoter and coding sequence, the {alpha} poly(A) site signals are utilized exclusively. These results show that the histone 3' end processing signals can function independently of the histone promoter and the transcripts which are normally polyadenylated ({alpha} globin) can be stabilized by a poly(A)– histone mRNA 3' terminus. Furthermore, these results show that the histone 3' end processing signals are less efficient than the {alpha} globin poly(A) site signals, if the two are placed in direct competition.


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