Nucleic Acids Research, 1986, Vol. 14, No. 19 7513-7528
© 1986
Articles |
In vitro processing of a plant pre-mRNA in a HeLa cell nuclear extract
Institut for Biochemie, Universitat Wien Wahringerstrasse 17, A-1090 Wien, Austria
*To whom correspondence should be addressed
Received August 18, 1986. Accepted September 12, 1986.
In order to determine whether there is a general difference in the splicing mechanism of animal and plant pre-mRNAs, we cloned part of the gene for the small subunit of the ribulose 1, 5-bisphosphate carboxylase containing both introns into the SP64 vector. RNA was synthesized with SP6 polymerase and used as substrate for in vitro processing in a HeLa cell nuclear splicing extract. Analyses of the processed RNA demonstrate that both introns of the plant pre-mRNA are efficiently removed in an ordered fashion yielding a faithfully ligated mRNA. Two branch points were identified for intron A and three for intron B. The branched nucleotides are adenosine residues in all cases and are located within a distance from the 3' splice site found to be crucial for lariat formation in animal pre-mRNAs.
The implications of these results are discussed in light of our previous observation, that a functional pre-mRNA of the human growth hormone gene was not processed in plant tissue in vivo (1).
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Golovkin and A. S. N. Reddy The Plant U1 Small Nuclear Ribonucleoprotein Particle 70K Protein Interacts with Two Novel Serine/Arginine –Rich Proteins PLANT CELL, October 1, 1998; 10(10): 1637 - 1648. [Abstract] [Full Text]
|
|