Skip Navigation

This Article
Right arrow Print PDF (1138K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (114)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Nedospasov, S. A.
Right arrow Articles by Jongeneel, C. V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nedospasov, S. A.
Right arrow Articles by Jongeneel, C. V.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1986, Vol. 14, No. 19 7713-7725
© 1986

Articles

The genes for lumor necrosis factor (TNF-alpha) and lymphotoxin (TNF-beta) are tandemly arranged on chromosome 17 of the mouse

S. A. Nedospasov1,2, B. Hirt1, A. N. Shakhov2, V. N. Dobrynin3, E. Kawashima4, R. S. Accolla5 and C. V. Jongeneel5,*

1Swiss Institute for Experimental Cancer Research CH-1066 Epalinges, Switzerland 2Institute of Molecular Biology, USSR Academy of Sciences Moscow, USSR 3Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences Moscow, USSR 4Biogen S. A. Route des Acacias 46, CH-1227 Carouge/Geneva 5Ludwig Institute for Cancer Research, Lausanne Branch CH-1066 Epalinges, Switzerland

*To whom correspondence should be addressed

Received July 31, 1986. Accepted September 9, 1986.

We have isolated clones containing the gene for tumor necrosis factor (TNF-alpha) from a mouse genomic library. Four out of five clones containing the TNF-alpha gene also hybridized to a human lymphotoxin (TNF-beta) probe. We constructed a restriction enzyme cleavage map of a 6.4 kb region from one of the genomic clones. From partial sequencing data and hybridizations with exon-specific oligonucleotide probes, we conclude that this region contains the mouse TNF-alpha and TNF-beta genes in a tandem arrangement, that they are separated by only about 1100 bases, and that their intron-exon structure is very similar to that seen in man. We probed genomic blots of DNA from human/mouse hybrids containing single mouse chromosomes for the presence of the mouse TNF genes. The results show that the genes are located on mouse chromosome 17, which also contains the major histocompatibi1ity complex. Therefore, both the mouse and the human TNF genes are tandemly arranged and located on the same chromosome as the MHC.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
A. V. Tsytsykova, R. Rajsbaum, J. V. Falvo, F. Ligeiro, S. R. Neely, and A. E. Goldfeld
Activation-dependent intrachromosomal interactions formed by the TNF gene promoter and two distal enhancers
PNAS, October 23, 2007; 104(43): 16850 - 16855.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
D. V. Kuprash23, M. B. Alimzhanov2, A. V. Tumanov2, A. O. Anderson, K. Pfeffer, and S. A. Nedospasov
TNF and Lymphotoxin {beta} Cooperate in the Maintenance of Secondary Lymphoid Tissue Microarchitecture But Not in the Development of Lymph Nodes
J. Immunol., December 15, 1999; 163(12): 6575 - 6580.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. L. Kiselev, O. S. Kustikova, E. V. Korobko, E. B. Prokhortchouk, A. A. Kabishev, E. M. Lukanidin, and G. P. Georgiev
Molecular Cloning and Characterization of the Mouse tag7 Gene Encoding a Novel Cytokine
J. Biol. Chem., July 17, 1998; 273(29): 18633 - 18639.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Med.Home page
D. Sean Riminton, H. Korner, D. H. Strickland, F. A. Lemckert, J. D. Pollard, and J. D. Sedgwick
Challenging Cytokine Redundancy: Inflammatory Cell Movement and Clinical Course of Experimental Autoimmune Encephalomyelitis Are Normal in Lymphotoxin-deficient, but Not Tumor Necrosis Factor-deficient, Mice
J. Exp. Med., May 4, 1998; 187(9): 1517 - 1528.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
J. R. Gruen and S. M. Weissman
Evolving Views of the Major Histocompatibility Complex
Blood, December 1, 1997; 90(11): 4252 - 4265.
[Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. K. Crawford, J. E. Ensor, I. Kalvakolanu, and J. D. Hasday
The Role of 3' Poly(A) Tail Metabolism in Tumor Necrosis Factor-alpha Regulation
J. Biol. Chem., August 22, 1997; 272(34): 21120 - 21127.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. B. Alimzhanov, D. V. Kuprash, M. H. Kosco-Vilbois, A. Luz, R. L. Turetskaya, A. Tarakhovsky, K. Rajewsky, S. A. Nedospasov, and K. Pfeffer
Abnormal development of secondary lymphoid tissues in lymphotoxin beta -deficient mice
PNAS, August 19, 1997; 94(17): 9302 - 9307.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
P De Togni, J Goellner, N. Ruddle, P. Streeter, A Fick, S Mariathasan, S. Smith, R Carlson, L. Shornick, J Strauss-Schoenberger, et al.
Abnormal development of peripheral lymphoid organs in mice deficient in lymphotoxin
Science, April 29, 1994; 264(5159): 703 - 707.
[Abstract] [PDF]

Home page
 ScienceHome page
A. Sinha, M. Lopez, and H. McDevitt
Autoimmune diseases: the failure of self tolerance
Science, June 15, 1990; 248(4961): 1380 - 1388.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.