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Nucleic Acids Research, 1986, Vol. 14, No. 2 1063-1072
© 1986

Articles

Revised assignment for the Bacillus subtilis spo0F gene and its homology with spo0A and with two Escherichia coli genes

Hirofumi Yoshikawa, Jun Kazami, Shinya Yamashita, Taku Chibazakura, Hidetaka Sone1, Fujio Kawamura, Masanao Oda2, Masami Isaka2, Yasuo Kobayashi2 and Hiuga Saito*

Institute of Applied Microbiology, University of Tokyo Bunkyo-ku, Tokyo 113, Japan 2Department of Applied Biochemistry, Hiroshima University Fukuyama 720, Japan

*To whom correspondence should be addressed

Received September 30, 1985. Revised December 3, 1985. Accepted December 3, 1985.

The nucleotide sequences of spo0F mutant genes which block the early sporulation process of Bacillus subtilis were determined. The mutation sites together with tEe results of complementation tests suggested that an open reading frame for a polypeptide of Mr=14,229 is the spo0F gene. The deduced amino acid sequence shows striking homology with that of the spo0A gene. In addition, the upstream region involving the ribosome binding site of the spo0F coding region is also similar to those of spo0A and spo0B. These homologies suggest that all three genes have a similar function in regulating the initiation of sporulation, and that their expression is controlled by a common mechanism. Clear homology is also seen between the spo0 gene products and the transcriptional control proteins, OmpR and Dye, of Escherichia coli suggesting that the spo0 gene products also are involved in the control of transcription.

1Present address: Kirin Brewery Co. Ltd. Brewing Science Laboratory, Takasaki 370-12, Japan


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