Skip Navigation

This Article
Right arrow Print PDF (2386K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Atmadja, J.
Right arrow Articles by Brimacombe, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Atmadja, J.
Right arrow Articles by Brimacombe, R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1986, Vol. 14, No. 2 659-673
© 1986

Articles

The tertiary folding of Escherichia coli 16S RNA, as studied by in situ intra-RNA cross-linking of 30S ribosomal subunits with bis-(2-chloroethyI)-methylamine

Johannes Atmadja, Wolfgang Stiege, Monica Zobawa, Barbara Greuer, Monika Osswald and Richard Brimacombe

Max-Planck-Institut für Molekulare Genetik Abteilung Wittmann, D-1000, Berlin-Dahlem, FRG

Received November 19, 1985. Accepted December 13, 1985.

Intra-RNA cross-links were introduced into E. coli 30S ribosomal subunits by treatment with bis-(2-chloroethyl)methylaraine. The subunits were partially digested with cobra venom nuclease, and the cross-linked complexes were separated by two-dimensional electrophoresis and analysed according to our published procedures. Tertiary structural cross-links in the 16S RNA were identified between nucleotides 31 and 306, and between the tetranucleotide 693–696 and nucleotides 794 or 799. Secondary structural cross-links, lying at the ends of double-helical regions, were found between nucleotides 46 and the trinucleotide 362–364, and between the dinucleotide 148–149 and nucleotide 174. Cross-links within double-helical elements were identified between the tetranucleotide 128–131 and nucleotide 232, between nucleotide 250 and the dinucleotide 274–275, and between nucleotides 1413 and 1486. Adenine as well as guanine residues were involved in the cross-links.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
J. W. Noah, T. Shapkina, and P. Wollenzien
UV-induced crosslinks in the 16S rRNAs of Escherichia coli, Bacillus subtilis and Thermus aquaticus and their implications for ribosome structure and photochemistry
Nucleic Acids Res., October 1, 2000; 28(19): 3785 - 3792.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
S Stern, T Powers, L. Changchien, and H. Noller
RNA-protein interactions in 30S ribosomal subunits: folding and function of 16S rRNA
Science, May 19, 1989; 244(4906): 783 - 790.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.