Nucleic Acids Research, 1986, Vol. 14, No. 2 751-763
© 1986
Articles |
Nonepidermal members of the keratin multigene family: cDNA sequences and in situ localization of the mRNAs
Institute of Experimental Pathology, German Cancer Research Center Im Neuenheimer Feld 280, 6900 Heidelberg, FRG
Received October 22, 1985. Accepted December 13, 1985.
A keratin set which consists of a type I 47kd and a type II 57kd protein occurs as a major constituent of the keratin patterns of various Internal stratified epithelia of the mouse. We have isolated specific cDNA clones of the two complementary keratin subunits from a cDNA library constructed with polyA+ RNA of mouse tongue epithelium and present the complete nucleo-t1de and deduced amino add sequences of the 57kd protein and about 75% of the corresponding data of the 47kd protein.
The comparison of the sequence data with those of known epidermal keratin mRNAs coding for the two types of keratin proteins reveals a fundamentally identical and type-specific organization of the mRNAs into both highly conserved and variable domains. In order to avoid cross-reactions wtth other members of the keratin multigene family, appropriately taylored35 S-labeled cDNA probes comprising the low and non-homologous 3' coding and noncoding domains of the mRNAs were used for in situ hybridization to tissue sections. The localization and distribution of the corresponding transcripts indicates a strongly compartmentalized keratin expression in mouse tongue epithelium.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  W.-W. Huang, Y. Yin, Q. Bi, T.-C. Chiang, N. Garner, J. Vuoristo, J. A. McLachlan, and L. Ma Developmental Diethylstilbestrol Exposure Alters Genetic Pathways of Uterine Cytodifferentiation Mol. Endocrinol., March 1, 2005; 19(3): 669 - 682. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Kikkawa, A. Oyama, R. Ishii, I. Miura, T. Amano, Y. Ishii, Y. Yoshikawa, H. Masuya, S. Wakana, T. Shiroishi, et al. A Small Deletion Hotspot in the Type II Keratin Gene mK6irs1/Krt2-6g on Mouse Chromosome 15, a Candidate for Causing the Wavy Hair of the Caracul (Ca) Mutation Genetics, October 1, 2003; 165(2): 721 - 733. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Toivola, Q. Zhou, L. S. English, and M. B. Omary Type II Keratins Are Phosphorylated on a Unique Motif during Stress and Mitosis in Tissues and Cultured Cells Mol. Biol. Cell, June 1, 2002; 13(6): 1857 - 1870. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. Ness, W. Edelmann, T. D. Jenkins, W. Liedtke, A. K. Rustgi, and R. Kucherlapati Mouse Keratin 4 Is Necessary for Internal Epithelial Integrity J. Biol. Chem., September 11, 1998; 273(37): 23904 - 23911. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Liao, N.-O. Ku, and M. B. Omary Stress, Apoptosis, and Mitosis Induce Phosphorylation of Human Keratin 8 at Ser-73 in Tissues and Cultured Cells J. Biol. Chem., July 11, 1997; 272(28): 17565 - 17573. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N.-O. Ku and M. B. Omary Phosphorylation of Human Keratin 8in Vivo at Conserved Head Domain Serine 23and at Epidermal Growth Factor-stimulated Tail Domain Serine 431 J. Biol. Chem., March 14, 1997; 272(11): 7556 - 7564. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Pang, A Schermer, J Yu, and T. Sun Suprabasal change and subsequent formation of disulfide-stabilized homo- and hetero-dimers of keratins during esophageal epithelial differentiation J. Cell Sci., January 3, 1993; 104(3): 727 - 740. [Abstract] [PDF]
|
|