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Nucleic Acids Research, 1986, Vol. 14, No. 2 899-914
© 1986

Articles

In vitro transcription of RU, a middle repetitive element of the rat genome

Guy Page, Maria Salvato1 and Howard M. Goodman2

Department of Applied Biological Sciences MIT 56-219, Cambridge, MA 02139, USA 1Scripps Clinic and Research Foundation 10666 N.Torrey Pines Rd, La Jolla, CA 92037, USA 2Department of Molecular Biology, Massachusetts General Hospital Boston, MA 02114, USA

Received May 17, 1985. Accepted December 9, 1985.

Repetitive DNA sequences have been found within as well as adjacent to the rat growth hornone (rGH) gene (1,2). In vitro transcription of the rGH gene yields transcripts predominantly from a tandemly duplicated repeat (RU) in the second intron of the gene. The relative {alpha}-amanitin sensitivity of this transcription indicates that it is carried out by RNA polymerase III. Transcription initiates within a 5' flanking 15 base-pair repeat, and terminates within a stretch of A residues at the end of each repeat, a termination site that is notably unlike aost of those used by polymerase III. Approximately 75% of the transcripts stop at the end of the first repeat; the remainder "read-through" to a homologous termination region at the end of the second repeat.


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