Nucleic Acids Research, 1986, Vol. 14, No. 2 965-982
© 1986
Articles |
Cloning of cDNA sequences of a progestin-regulated mRNA from MCF7 human breast cancer cells
Unité d'Endocrinologie Cellulaire et Moléculaire, U 148 INSERM, 60, rue de Navacelles, 34100 Montpellier, France
Received November 12, 1985. Accepted December 5, 1985.
A cDNA clone corresponding to an mRNA regulated by the progestin R5020, has been isolated by differential screening of a cDNA library from the MCF7 breast cancer cell line, which contains estrogen and progesterone receptors. This probe hybridized with a single species of poly A + RNA of 8-kb molecular weight as shown by Northern blot analysis and could also be used to total RNA preparation. This recombinant clone hybridized specifically to an mRNA coding for a 250,000 daltons protein when tranlated in vitro. This protein was identical to the 250 kDa progestin-regulated protein that. we previously described (Biochem. Biophys. Res. Commun. 121, 421–427, 1984) as shown by immunoprecipitation with specific rabbit polyclonal antibodies. Dose-response curve and specificity studies show that the accumulation of the Pg8 mRNA and that of the 250-kDa protein was increased by 5 to 30-fold following progestin treatment and that this effect was mediated by the progesterone receptor. Time course of induction indicated that the accumulation of mRNA was rapid and preceded that of the protein. This is the first report on a cloned cDNA probe of progestin-regulated mRNA in human cell lines.
* Present address : Department of Biochemistry, Ridley Building, The University, New Castle Upon Tyne NE1 7RU England.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Bardin, F. Moll, R. Margueron, C. Delfour, M. L. Chu, T. Maudelonde, V. Cavailles, and P. Pujol Transcriptional and Posttranscriptional Regulation of Fibulin-1 by Estrogens Leads to Differential Induction of Messenger Ribonucleic Acid Variants in Ovarian and Breast Cancer Cells Endocrinology, February 1, 2005; 146(2): 760 - 768. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Bardin, P. Hoffmann, N. Boulle, D. Katsaros, F. Vignon, P. Pujol, and G. Lazennec Involvement of Estrogen Receptor {beta} in Ovarian Carcinogenesis Cancer Res., August 15, 2004; 64(16): 5861 - 5869. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. M. McGowan and C. L. Clarke Effect of Overexpression of Progesterone Receptor A on Endogenous Progestin-Sensitive Endpoints in Breast Cancer Cells Mol. Endocrinol., October 1, 1999; 13(10): 1657 - 1671. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. K. Richer, C. A. Lange, N. G. Manning, G. Owen, R. Powell, and K. B. Horwitz Convergence of Progesterone with Growth Factor and Cytokine Signaling in Breast Cancer. PROGESTERONE RECEPTORS REGULATE SIGNAL TRANSDUCERS AND ACTIVATORS OF TRANSCRIPTION EXPRESSION AND ACTIVITY J. Biol. Chem., November 20, 1998; 273(47): 31317 - 31326. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. F. Beers, K. O. Solarin, S. H. Guttentag, J. Rosenbloom, A. Kormilli, L. W. Gonzales, and P. L. Ballard TGF-beta 1 inhibits surfactant component expression and epithelial cell maturation in cultured human fetal lung Am J Physiol Lung Cell Mol Physiol, November 1, 1998; 275(5): L950 - L960. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Graham and C. L. Clarke Physiological Action of Progesterone in Target Tissues Endocr. Rev., August 1, 1997; 18(4): 502 - 519. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. A. Kester, B.-j. M. van der Leede, P. T. van der Saag, and B. van der Burg Novel Progesterone Target Genes Identified by an Improved Differential Display Technique Suggest That Progestin-induced Growth Inhibition of Breast Cancer Cells Coincides with Enhancement of Differentiation J. Biol. Chem., June 27, 1997; 272(26): 16637 - 16643. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Kalkhoven, S. Wissink, P. T. van der Saag, and B. van der Burg Negative Interaction between the RelA(p65) Subunit of NF-kappaB and the Progesterone Receptor J. Biol. Chem., March 15, 1996; 271(11): 6217 - 6224. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Lacasa, X. Le Liepvre, P. Ferre, and I. Dugail Progesterone Stimulates Adipocyte Determination and Differentiation 1/Sterol Regulatory Element-binding Protein 1c Gene Expression. POTENTIAL MECHANISM FOR THE LIPOGENIC EFFECT OF PROGESTERONE IN ADIPOSE TISSUE J. Biol. Chem., April 6, 2001; 276(15): 11512 - 11516. [Abstract] [Full Text] [PDF]
|
|