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Nucleic Acids Research, 1986, Vol. 14, No. 20 7915-7927
© 1986

Articles

Tn7 transposition: a multigene process, Identification of a regulatory gene product

Gillian M. Smith* and Peter Jones1

ICI/University Joint Laboratory Adrian Building, University Road, Leicester LE1 7RH, UK 1Department of Biochemistry, School of Biological Sciences, University of Leicester Adrian Building, University Road, Leicester LE1 7RH, UK

*To whom correspondence should be addressed

Received July 4, 1986. Accepted September 24, 1986.

Tn7 transposition is abolished by the deletion of a 2.2kb Hindlll fragment froa the central region of the transposon. Transposition is restored when the fragment is present in trans. When this fragment is present in trans with wild-type Tn7, transposition frequencies are stimulated 10–100-fold. The DNA sequence of this fragment has been determined and found to contain one long open reading frame coding for a protein of molecular weight 61 187. We have visualised this protein using a DNA-directed prokaryotlc transcription-translation system. This gene may fill a regulatory role in the mechanism of Tn7 transposition. When present in trans the 2.2kb Hindlll fragment alleviates a tranacriptional block of pronoter activity detected in Tn7.


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