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Nucleic Acids Research, 1986, Vol. 14, No. 20 8183-8188
© 1986

Articles

Two or more copies of Drosophila heat shock consensus sequence serve to activate transcription in yeast

Randolph Wei, Hilary Wilkinson, Karl Pfeifer, Carrie Schneider, Richard Young and Leonard Guarente

Department of Biology, Massachusetts Institute of Technology Cambridge, MA 02139, USA

Received May 21, 1986. Accepted September 25, 1986.

A synthetic oligonucleotide bearing the Drosophila heat shock consensus sequence confers heat inducibility on a CYC1-lacZ gene in Saccharomvces cerevisiae. This sequence CTGGAATTTTCTAGA was inserted in place of the upstream activation sites of the CYC1 promoter adjacent to CYC1 TATA boxes. These constructs were transformed into yeast and found to be heat-inducible when two or more inserts were present. The level of inducibility seemed to increase with the number of inserted sequences: however, the orientations of these sequences relative to each other did not have much effect.


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N. Hashikawa and H. Sakurai
Phosphorylation of the Yeast Heat Shock Transcription Factor Is Implicated in Gene-Specific Activation Dependent on the Architecture of the Heat Shock Element
Mol. Cell. Biol., May 1, 2004; 24(9): 3648 - 3659.
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