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Nucleic Acids Research, 1986, Vol. 14, No. 20 8189-8207
© 1986

Articles

Replication origins are attached to the nuclear skeleton

S. V. Razin*, M. G. Kekelidze+, E. M. Lukanidin, K. Scherrer1 and G. P. Georgiev

Institute of Molecular Biology, USSR Academy of Sciences 117984 Moscow B-334, USSR 1Service de Biochemie de la Différentiation, Institut de Recherche en Biologie Moléculaire 75251 Paris, Cedex 05, France

Received May 9, 1986. Accepted September 12, 1986.

DNA fragments containing replication origins (oriDNA) were isolated from a chicken erythroblast cell line by a modified procedure of Zannis-Hadjopoulos et al. /1, 2/ and studied in the renaturation reaction driven by either total or nuclear matrix DNA (nmDNA) from the same cells or from mature erythrocytes. We found that the unique sequences of nmDNA from erythroblasts (5 kb long) represented a specific subset of sequences constituting about a quarter of total DNA unique sequences, while the erythrocyte nmDNA 5 kb fragments constitute only about one tenth of total unique DNA and all are recovered among erythroblast nmDNA. Virtually all oriDNA sequences are present in the fraction of erythrocyte nmDNA. Thereafter, the putative positions of replication origins within the {propto} -globine gene domain have been mapped by hybridization experiments. They were found to coincide with the previously established positions of permanent sites of DNA attachment to the nuclear matrix.

+Present Address: Institute of Pysiology, Georgian Academy of Sciences, Tblissi 380060, USSR

*To Whom reprint requests to be sent


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