Skip Navigation

This Article
Right arrow Print PDF (1621K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Portugal, J.
Right arrow Articles by Waring, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Portugal, J.
Right arrow Articles by Waring, M. J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1986, Vol. 14, No. 22 8735-8754
© 1986

Articles

Antibiotics which can alter the rotational orientation of nucleosome core DNA

José Portugal and Michael J. Waring

Department of Pharmacology, University of Cambridge Medical School Hills Road, Cambridge CB2 2QD, UK

Received September 19, 1986. Accepted October 16, 1986.

Four well-characterised DNA-binding ligands have been tested for effects on reconstituted nucleosome core particles containing the 160 bp tyrT DNA fragment. Two, netropsin and berenil, were found to change the rotational orientation of the DNA on the surface of the protein as judged by marked alterations in the pattern of fragments produced by exposure to DNAase I. Qualitatively their effects were very similar to those previously reported for the related antibiotic distamycin, suggesting that the phenomenon of induced rotation may be a characteristic property of ligands which bind in the narrow groove of the DNA helix. Two intercalators did not produce the effect but, at high concentrations, caused gross disruption of the nucleoprotein structure with apparent release of DNA from the histone octamer. At moderate concentrations little or no effect was detectable with nogalamycin, suggestive of failure to bind as a result of constraints on local opening of the DNA helix. With moderate concentrations of actinomycin, protection of GpC sequences was clearly visible together with some evidence of increase in helix pitch, but no sign of altered phasing of DNA within the nucleosome core particles.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
T. D. Schaal, W. G. Mallet, D. L. McMinn, N. V. Nguyen, M. M. Sopko, S. John, and B. S. Parekh
Inhibition of human papilloma virus E2 DNA binding protein by covalently linked polyamides
Nucleic Acids Res., February 15, 2003; 31(4): 1282 - 1291.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Y. Hsu and Y.-H. Wang
Human Fragile Site FRA16B DNA Excludes Nucleosomes in the Presence of Distamycin
J. Biol. Chem., May 3, 2002; 277(19): 17315 - 17319.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.