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Nucleic Acids Research, 1986, Vol. 14, No. 22 8963-8977
© 1986

Articles

AnalysIs of the primary structure and promoter function of a pyruvate decarbozylase gene (PDCI) from Saceharomyces cerevisiae

Elke Kellermann, Peter G. Seeboth and Cornelis P. Hollenberg

Institut für Mikrobiologie, Universität Düsseldorf, Universitätsstrasse 1 4000 Düsseldorf 1, FRG

Received July 4, 1986. Revised October 23, 1986. Accepted October 31, 1986.

The PDC1 gene of Saccharomyces cerevisiae, encoding pyruvate decartoxylase was sequenced. The gene contains an cen reaiing frame of 1647 base pairs. The codon usage shows the same strong bias as fcur1 for some other glycolytic enzymes. Transcription starts mainly at –30 and terminates 100 base pairs downstream of the termination codon. In some strains a second termination site, 46 base pairs upatream of the stcp codon was oheerved.

The function of the promoter region was analyzed by fusion to the bacterial structural gene encoding {ell}-lactamase (bla. C multicopy plasmid or integrated in the genome, the expression of the bla gene showed the regulation of the authentic PDC1 gene.


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