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Nucleic Acids Research, 1986, Vol. 14, No. 23 9353-9370
© 1986

Articles

The nucleotide sequence and transcription of miniclrcular mitochondrial DNA's associated with male-fertile and cytoplasmic male-sterile lines of sugarbeet

Colwyn M. Thomas

Department of Applied Genetics, John Innes Institute Colney Lane, Norwich NR4 7UH, UK

Received September 23, 1986. Accepted October 31, 1986.

Male-fertile (MF) and cvtonlasmic male-sterile (CMS) lines of sugarbeet possess characteristic low molecular weight minicircular mitochondrial DNA's, and Southern hybridizations revealed seauence homolofcies between them. The complete nucleotide sequences of mlnicircle a present in MF and CMS lines, and minicircle d present in some MF lines have been determined and compared to mlnicircle c present in all MF lines. Computer analysis revealed seauences common to all three DNA's: these may be essential to their maintenance in sugarbeet mitochondria. Analysis of the extent and arrangement of homologous seauences in minicircle a and minicircle d suggests the latter may have arisen from an intramolecular recombination event in minicircle a. The G+C contents of the minicircles differ and their possible origins are discussed. All three DNA's are transcribed. Minicircle c and ralnicircle d transcripts are exclusive to MF mitochondria, but are unlikelv to encode protein products. Two transcripts of sufficient size and the predicted rtolaritv to encompass a short open reading frame on minicircle a are common to both MF and CMS mitochondria.


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