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Nucleic Acids Research, 1986, Vol. 14, No. 3 1209-1217
© 1986

Articles

A human gastric carcinoma contains a single mutated and an amplified normal allele of the Ki-ras oncogene

J.L. Bos, M.Verlaan-de Vries, C.J. Marshall1, G.H. Veeneman2, J.H. van Boom2 and A.J. van der Eb

Department of Medical Biochemistry, Sylvius Laboratories, State University of Leiden P.O. Box 9503, 2300 RA Leiden, The Netherlands 1Section of Cell and Molecular Biology, Chester Beatty Laboratories, Institute of Cancer Research Fulham Road, London SW3 6JB, UK 2Organic Chemistry Laboratories, Gorlaeus Laboratories P.O. Box 9502, 2300 RA Leiden, The Netherlands

Received November 26, 1985. Revised January 14, 1986. Accepted January 14, 1986.

The DNA from various human tumors and tumor cell lines was screened for the presence of mutated ras oncogenes with synthetic oligonucleotide probes, as well as with the NIH/3T3 cell transfection assay. Among the various mutations found we discovered two novel Ki-ras mutations in codon 12: gly to ala and gly to ser. A gastric carcinoma was found to possess a single mutated Ki-ras allele (gly-12 to ser), as well as a 30–50 fold amplified normal allele. This implies that two activating steps must have occurred in this malignancy.


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