Improvement of the dideoxy chain termination method of DNA sequencing by use of deoxy-7-deazaguanosine triphosphate in place of dGTP

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Nucleic Acids Research, 1986, Vol. 14, No. 3 1319-1324
© 1986

Articles

Improvement of the dideoxy chain termination method of DNA sequencing by use of deoxy-7-deazaguanosine triphosphate in place of dGTP

Saeko Mizusawa, Susumu Nishimura^* and Frank Seela⁺

⁺Laboratory of Bioorganic Chemistry, Department of Chemistry, University of Paderborn D-4790 Paderborn, FRG Biology Division, National Cancer Center Research Institute Chuo-ku, Tokyo, Japan

^*To whom reprint requests should be sent

Received October 16, 1985. Revised January 6, 1986. Accepted January 6, 1986.

The dideoxy chain termination method using deoxy-7-deaza-guanosinetriphosphate (dc⁷GTP)in place of dGTP was found to be very useful.Sequencing of a part of the human N-myc gene having 85% GC contentis impossible by the original method using dGTP, because ofcompression of bands. However, the nucleotide sequence of thispart was unambiguously determined by analysis of both strandsby the modified method. Use of dc⁷GTP is concluded to improvethe dideoxy chain termination method for DNA sequencing.

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