Skip Navigation

This Article
Right arrow Print PDF (1824K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Hossle, J.P.
Right arrow Articles by Perriard, J.-C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hossle, J.P.
Right arrow Articles by Perriard, J.-C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1986, Vol. 14, No. 3 1449-1463
© 1986

Articles

The primary structure of chicken B-creatine kinase and evidence for heterogeneity of its mRNA

J.P. Hossle, U.B. Rosenberg*, B. Schäfer, H.M. Eppenberger and J.-C. Perriard**

Institute for Cell Biology, Swiss Federal Institute of Technology CH-8093 Zürich, Switzerland

**To whom correspondence and reprint request should be addressed.

Received October 30, 1985. Revised January 2, 1986. Accepted January 2, 1986.

cDNA clones for chicken B-CK were isolated by immunoscreening from a gizzard cDNA library constructed in the expression vector {lambda}gtll. The entire coding portion in addition to the complete 3' untranslated region and 42 bp of the 5' noncoding part are represented in the clone H4. On RNA blots H4 insert DNA hybridized to a 1600 bp poly(A)+ RNA from gizzard, brain and heart but not to breast or skeletal muscle RNA. In vitro generated sense strand transcripts of H4 insert DNA were translated in vitro into a protein indistinguishable from isolated, authenti c B-CK. The distinct nucleotide sequences of H4 insert DNA and M-CK cDNA were translated into 82% homologous amino acid sequences. Sequence heterogeneity among the B-CK cDNA clones within both the 3' noncoding and even in the coding region indicates the existence of multiple B-CK mRNA species.

* Current address: Lonza AG, CH-393O Visp, Switzerland.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 JCBHome page
T. Hornemann, M. Stolz, and T. Wallimann
Isoenzyme-Specific Interaction of Muscle-Type Creatine Kinase with the Sarcomeric M-Line Is Mediated by Nh2-Terminal Lysine Charge-Clamps
J. Cell Biol., June 12, 2000; 149(6): 1225 - 1234.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H.-W. Sun, C.-F. Hui, and J.-L. Wu
Cloning, Characterization, and Expression in Escherichia coli of Three Creatine Kinase Muscle Isoenzyme cDNAs from Carp (Cyprinus carpio) Striated Muscle
J. Biol. Chem., December 11, 1998; 273(50): 33774 - 33780.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M Stolz and T Wallimann
Myofibrillar interaction of cytosolic creatine kinase (CK) isoenzymes: allocation of N-terminal binding epitope in MM-CK and BB-CK
J. Cell Sci., January 5, 1998; 111(9): 1207 - 1216.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
R. J. Denver, S. Pavgi, and Y.-B. Shi
Thyroid Hormone-dependent Gene Expression Program for Xenopus Neural Development
J. Biol. Chem., March 28, 1997; 272(13): 8179 - 8188.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Bantle, S. Keller, I. Haussmann, D. Auerbach, E. Perriard, S. Muhlebach, and J.-C. Perriard
Tissue-specific Isoforms of Chicken Myomesin Are Generated by Alternative Splicing
J. Biol. Chem., August 9, 1996; 271(32): 19042 - 19052.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. M. Mühlebach, T. Wirz, U. Brändle, and J.-C. Perriard
Evolution of the Creatine Kinases
J. Biol. Chem., May 17, 1996; 271(20): 11920 - 11929.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.