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Nucleic Acids Research, 1986, Vol. 14, No. 5 2201-2213
© 1986

Articles

The isolation and nucleotide sequence of the complex AROM locus of Aspergillus nidulans

Ian G. Charles1, John W. Keyte1, William J. Brammar1, Melanie Smith2 and Alastair R. Hawkins2,1,*

1Department of Biochemistry, University of Leicester Leicester LEI 7RH 2Department of Genetics, Ridley Building, University of Newcastle upon Tyne Newcastle upon Tyne NE1 7RU, UK

*To whom correspondence should be sent at the University of Newcastle upon Tyne

Received December 30, 1985. Revised February 10, 1986. Accepted February 10, 1986.

The AROM locus of A.nldulans, which governs five consecutive step3 in pre-chorismate aromatic amino acid biosynthesis, ha3 been cloned in a bacterlophage vector. The nucleotlde sequence of the locus reveals a single, open reading-frame of 4,812 base-pairs, apparently without introna. An Internal segment of the A.nldulan3 AROM sequence has extensive homology with the E.coli aroA gene that encodes the 5-enolpyruvylshlkimate 3-phosphate synthase.


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