Skip Navigation

This Article
Right arrow Print PDF (687K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (95)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Schofield, P. R.
Right arrow Articles by Watson, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schofield, P. R.
Right arrow Articles by Watson, J. M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1986, Vol. 14, No. 7 2891-2903
© 1986

Articles

DNA sequence of Rhizobium trifolii nodulation genes reveals a reiterated and potentially regulatory sequence preceding nodABC and nodFE

Peter R. Schofield1 and John M. Watson*

CSIRO, Division of Plant Industry GPO Box 1600, Canberra, ACT 2601, Australia

*To whom correspondence should be addressed

Received February 13, 1986. Accepted March 13, 1986.

The Rhizobium trifolii nod genes required for host–specific nodulation of clovers are located on 14 kb of Sym (symbiotic) plasmid DNA. Analysis of the nucleotide sequence of a 3.7 kb portion of this region has revealed open reading frames corresponding to the nodABCDEF genes. A DNA sequencing technique, using primer extension from within Tn 5, has been used to determine the precise locations of Tn 5 mutations within the nod genes and the phenotypes of the corresponding mutants correlate with their mapped locations. The predicted nodA and nodB genes overlap by four nucleotides and the nod F and nodE genes overlap by a single nucleotide, suggesting that translational coupling may ensure the synthesis of equimolar amounts of these gene products. ThenodABC and nodFE genes constitute separate transcriptional units and each is preceded by a conserved 76–bp sequence which may be involved in the regulation of expression of these genes.

1Present address: Department of MOlecular Biology, Genentech, Inc., 460 Point San Bruno Boulevard, South San Francisco, CA 94080,USA


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Bacteriol.Home page
M. C. Peck, R. F. Fisher, and S. R. Long
Diverse Flavonoids Stimulate NodD1 Binding to nod Gene Promoters in Sinorhizobium meliloti.
J. Bacteriol., August 1, 2006; 188(15): 5417 - 5427.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
G. Epple, K. M. G. M. van der Drift, J. E. Thomas-Oates, and O. Geiger
Characterization of a Novel Acyl Carrier Protein, RkpF, Encoded by an Operon Involved in Capsular Polysaccharide Biosynthesis in Sinorhizobium meliloti
J. Bacteriol., September 15, 1998; 180(18): 4950 - 4954.
[Abstract] [Full Text]

Home page
 Genes Dev.Home page
R F Fisher, T T Egelhoff, J T Mulligan, and S R Long
Specific binding of proteins from Rhizobium meliloti cell-free extracts containing NodD to DNA sequences upstream of inducible nodulation genes.
Genes & Dev., March 1, 1988; 2(3): 282 - 293.
[Abstract] [PDF]

Home page
 Genes Dev.Home page
J. Burn, L. Rossen, and A.W.B. Johnston
Four classes of mutations in the nodD gene of Rhizobium leguminosarum biovar. viciae that affect its ability to autoregulate and/or activate other nod genes in the presence of flavonoid inducers
Genes & Dev., July 1, 1987; 1(5): 456 - 464.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.