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Nucleic Acids Research, 1986, Vol. 14, No. 7 3059-3073
© 1986

Articles

Isolation, physical characterization and expression analysis of the Saccharomyces cerevisiae positive regulatory gene PH04

M. Legrain, M. De Wilde1 and F. Hilger*

Laboratoire de Microbiologie, Facultè des Sciences Agronomiques B 5800 Gembloux 1Molecular Genetics, Smith Kline–RIT B 1330 Rixensart, Belgium

*To whom reprint requests should be sent

Received January 29, 1986. Accepted March 11, 1986.

The Saccharomyces cerevisiae PH04 gene, which positively controls the expression of phosphatase genes, has been isolated by complementation of a PH04 mutation. The Isolated DNA directed integration at the chromosomal PH04 locus. The nucleotide sequence of PH04 has a coding region of 930 nucleotides, flanked by sequences with typical transcription initiation and termination signals. The 5' region has characteristics of low–expression promoters and carries several uncommon elements, whose significance is not known. The predicted primary structure of the PH04 protein, of 309 residues, does not show sequence elements typical of DNA–binding proteins. The transcription of PH04 is independent of inorganic phosphate. Like other regulatory genes, PH04 is transcribed at a very low level and the translation of its message uses preferentially several codons which are not employed for highly expressed genes.


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