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Nucleic Acids Research, 1986, Vol. 14, No. 8 3153-3166
© 1986

Articles

Processing in the external transcribed spacer of ribosomal RNA from Physarum polycephalum

Beat Blum, Gerard Pierron1, Thomas Seebeck and Richard Braun

Institut für Allgemeine Mikrobiologie, University of Bern Baltzerstrasse 4, 3012 Bern, Switzerland 1Institut de Recherches scientifiques sur le Cancer BP no 8, 94802 Villejuif, Cedex, France

Received March 19, 1986. Accepted April 2, 1986.

The rDNA of the myxomycete Physarum polycephalum is transcribed to give a 13.3 kb precursor of ribosomal RNA. At 1.7kb downstream of the primary initiation site there is a processing site or a second initiation site. This site was studied by S1-mapping, DNA sequencing and electron microscopy. None of these methods could conclusively distinguish between the two formal possibilities. However, capping experiments indicate that rapid processing is taking place at this site rather than reinitiation. In addition, primary transcripts and processed molecules were assayed throughout the synchronous mitotic cycle. During all interphase stages newly initiated transcripts of rDNA and products of the first processing step are present in similar amounts, indicating control of initiation and not of maturation as being the main regulatory step for the accumulation of mature rRNAs. During the brief period of mitosis the level of newly initiated rRNA precursors is lowered.


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