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Nucleic Acids Research, 1986, Vol. 14, No. 8 3293-3310
© 1986

Articles

Effect of trypsinization and histone H5 addition on DNA twist and topology in reconstituted minichromosomes

Randall H. Morse and Charles R. Cantor

Department of Human Genetics, Columbia University College of Physicians and Surgeons 701 W. 168th St., New York, NY 10032, USA

Received December 16, 1985. Revised March 18, 1986. Accepted March 18, 1986.

Free DNA in solution exhibits an untwisting of the double helix with increasing temperature. We have shown previously that when DNA is reconstituted with histones to form nucleosome core particles, both the core DNA and the adjacent linker DNA are constrained from thermal untwisting. The origin of this constraint is unknown. Here we examine the effect of two modifications of nucleosome structure on the constraint against thermal untwisting, and also on DNA topology. In one experiment, we removed the highly positively charged histone amino and carboxy termini by trypsinization. Alternatively, we added histone H5, a histone H1 variant from chick erythrocytes. Neither of these modifications had any major effect on DNA topology or twist in the nucleosome.


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