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Nucleic Acids Research, 1986, Vol. 14, No. 8 3363-3376
© 1986


Articles

Isolation of oligonucleosomes from active chromatin using HMG17-specific monoclonal antibodies

Tomislav Dorbic and Burghardt Wittig*

Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Arnimallee 22, D–1000 Berlin 33, FRG

*To whom all scientific correspondence should be sent

Received December 9, 1985. Revised March 17, 1986. Accepted March 17, 1986.

We report the preparation of HM017-containing oligonucleosomes from chicken embryos and from liver and oviduct of laying hens. Monoclonal antibodies against HM017 were used for their isolation. An unusual size distribution with respect to their repeat number was observed. The oligonucleosomes of repeat number up to N6 were highly enriched for DNA of the vitellogenin II gene (liver) and for DNA of the ovalbumin and lysozyme genes (oviduct).


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