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Nucleic Acids Research, 1986, Vol. 14, No. 8 3475-3485
© 1986

Articles

A ‘hot-spot’ for Ty transposition on the left arm of yeast chromosome III

John R. Warmington, Rashida Anwar, Carol S. Newlon1, Richard B. Waring2, R. Wayne Davies3, Keith J. Indge and Stephen G. Oliver*

Department of Biochemistry and Applied Molecular Biology, University of Manchester Institute of Science and Technology PO Box 88, Manchester M60 1QD, UK 1Department of Microbiology UMDNJ - New Jersey Medical School 100 Bergen Street, Newark, NJ 07103, USA

*To whom correspondence should be addressed

Received February 12, 1986. Revised March 27, 1986. Accepted March 27, 1986.

The small ring derivative of Saccharomyces cerevisiae chromosome III, which was formed by a cross-over between HML on the left arm and HMR on the right arm, contains three Ty elements. The class II element Ty 1-17 lies immediately centromere-distal to LEU2 on the left arm while two class I elements are tandemly arranged distal to PGK on the right arm. We have sequenced the regions of chromosome III surrounding Ty 1-17 and have defined a region where a number of transposition events have occurred. This region is flanked by the 5' ends of two tRNA genes, tRNA3Glu on the centromere distal side and tRNA3Leu immediately in front of LEU2. Close to the tRNA3Glu gene there is a region containing degenerate delta sequences organised in opposite orientations. Immediately distal to Ty 1-17 there are two complete solo delta elements, one inserted into the other. The sequence indicates that these two delta sequences were inserted into chromosome III by separate transposition events. A model is presented to explain how this structure arose and the role of solo delta elements in transposon propagation and maintenance is discussed.

2Present address: Department of Parmacology, UMDNJ, Rutgers Medical School Piscataway, NJ 08854, USA

3Present address: Allelix Inc., 6850 Goreway Drive, Mississauga, Ontario L4V IPI, Canada


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