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Nucleic Acids Research, 1987, Vol. 15, No. 1 141-160
© 1987

Articles

Unusual features of transcribed and translated regions of the histone H4 gene family of Tetrahymena thermophila

Stuart Horowitz1, Josephine K. Bowen, Gary A. Bannon2 and Martin A. Gorovsky*

Department of Biology, University of Rochester Rochester, NY 14627, USA

*To whom correspondence should be addressed

Received August 18, 1986. Revised October 27, 1986. Accepted November 17, 1986.

The complete DNA sequence is presented of H4-II, the second of the pair of histone H4 genes of the ciliated protozoan, Tetrahymena thermophila. Both H4 genes code for the same protein. Codon usage 1n these and other Tetrahymena genes is severely restricted and is similar to that in yeast. Flanking regions are AT-rich (>75%), relative to coding sequences (~45% GC). Except for small, similarly positioned homologies, flanking sequences of the two genes are different. Canonical sequences in higher eukaryotic promoters are not obvious in these genes. Instead, short, localized, base composition eccentricities characterize the 5' flanking sequences of all Tetrahymena genes analyzed. The consensus, P yP u(A)3–4 ATGG initiates translation in these and all other known Tetrahymena genes. Nuclear transcripts and messages of bot growing and starved cells begin at multiple sites, mainly at the first or second A residue following a pyrimidine. The palindrome typical of histone message 3' termini in higher organisms is not present. Downstream of both genes are sequences similar to the processing/polyadenylation signal of higher eukaryotes, although the unique 3' ends are not those predicted by the location of the signals.

1Present addresses: 1Department of Pediatrics, University of Rochester, Rochester, NY 14627

2Present addresses: 2Department of Biochemistry, University of Arkansas, Little Rock, AR 72205, USA


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