Skip Navigation

This Article
Right arrow Print PDF (4824K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (49)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Dobner, P. R.
Right arrow Articles by Villa-Komaroff, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dobner, P. R.
Right arrow Articles by Villa-Komaroff, L.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1987, Vol. 15, No. 1 199-218
© 1987

Articles

Alternative 5' exons either provide or deny an initiator methionine codon to the same {alpha}-tubulin coding region

Paul R. Dobner, Edward Kislauskis, Bruce M. Wentworth+ and Lydia Villa-Komaroff+

University of Massachusetts Medical Center, Department of Molecular Genetics and Microbiology, 55 Lake Avenue North Worcester, MA 01605, USA

Received July 9, 1986. Revised December 4, 1986. Accepted December 4, 1986.

The primary structures of two overlapping novel {alpha}-tubulin cDNA clones isolated from a Macaca fascicularis testis cDNA library and the corresponding numan gene are presented. Although the general structure of the human gene conforms to that of previously described mammalian {alpha}-tubulin genes, there is a surprising difference: the ATG initiator codon is conspicuously absent. The macaque testis cDNA similarly lacks the initiator methionine, but otherwise encodes a variant {alpha}-tubulin isotype precisely conserved in the human gene. RNA blot analysis in the macaque, using a 3' untranslated region probe, revealed the existence of two additional related transcripts expressed in every tissue examined except the adult testis. Sequence comparisons indicate that the 2.0 kb testis transcript and one of the additional transcripts result from differential transcription of the same gene. The two transcripts differ only at the 5' end as a result of the recruitment of different 5' exons. Curiously, the 5' exon utilized outside the testis encodes an initiator methionine in the expected location.

+Present address: Department of Neuroscience, The Children's Hospital, 300 Longwood Avenue, Boston, MA 02115, USA


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 JCOHome page
C. Dumontet and B. I. Sikic
Mechanisms of Action of and Resistance to Antitubulin Agents: Microtubule Dynamics, Drug Transport, and Cell Death
J. Clin. Oncol., March 1, 1999; 17(3): 1061 - 1061.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.