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Nucleic Acids Research, 1987, Vol. 15, No. 1 199-218
© 1987

Articles

Alternative 5' exons either provide or deny an initiator methionine codon to the same {alpha}-tubulin coding region

Paul R. Dobner, Edward Kislauskis, Bruce M. Wentworth+ and Lydia Villa-Komaroff+

University of Massachusetts Medical Center, Department of Molecular Genetics and Microbiology, 55 Lake Avenue North Worcester, MA 01605, USA

Received July 9, 1986. Revised December 4, 1986. Accepted December 4, 1986.

The primary structures of two overlapping novel {alpha}-tubulin cDNA clones isolated from a Macaca fascicularis testis cDNA library and the corresponding numan gene are presented. Although the general structure of the human gene conforms to that of previously described mammalian {alpha}-tubulin genes, there is a surprising difference: the ATG initiator codon is conspicuously absent. The macaque testis cDNA similarly lacks the initiator methionine, but otherwise encodes a variant {alpha}-tubulin isotype precisely conserved in the human gene. RNA blot analysis in the macaque, using a 3' untranslated region probe, revealed the existence of two additional related transcripts expressed in every tissue examined except the adult testis. Sequence comparisons indicate that the 2.0 kb testis transcript and one of the additional transcripts result from differential transcription of the same gene. The two transcripts differ only at the 5' end as a result of the recruitment of different 5' exons. Curiously, the 5' exon utilized outside the testis encodes an initiator methionine in the expected location.

+Present address: Department of Neuroscience, The Children's Hospital, 300 Longwood Avenue, Boston, MA 02115, USA


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