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Nucleic Acids Research, 1987, Vol. 15, No. 1 267-275
© 1987

Articles

Assignment of the 13C nuclear magnetic resonance spectrum of a short DNA-duplex with 1H-detected two-dimensional heteronuclear correlation spectroscopy

Werner Leupin, Gerhard Wagner, William A. Denny1 and Kurt Wüthrich

1Cancer Research Laboratory, University of Auckland School of Medicine Private Bag Auckland, New Zealand Institut für Molekularbiologie und Biophysik, ETH-Hönggerberg CH-8093 Zürich, Switzerland

Received November 4, 1986. Accepted December 1, 1986.

Proton-detected 1H-13C heteronuclear correlated spectroscopy ([1H,13C]-COSY) was used to establish relations between the carbon-13 and proton nuclear magnetic resonance chemical shifts in the hexadeoxynucleoside penta-phosphate d-(GCATGC)2. Using the previously established sequence-specific proton NMR assignments, sequence-specific assignments were thus obtained for nearly all proton-bearing carbons. This approach offers a new criterion for distinguishing between the proton NMR lines of purines and pyrimidines, based on the different proton-carbon-13 coupling constants. Furthermore, the adenine ring carbon 2 has a unique carbon-13 chemical shift, which enables a straightforward identification of the adenine C2H resonances by [1H,13C]-COSY.


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