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Nucleic Acids Research, 1987, Vol. 15, No. 1 87-103
© 1987

Articles

Replication of proto-oncogenes early during the S phase in mammalian cell lines

Mohammed A. Iqbal1, Jeffrey Chinsky2, Valerie Didamo and Carl L. Schildkraut1,*

1Department of Cell Biology Albert Einstein College of Medicine Bronx, NY 10461, USA 2Department of Genetics, Albert Einstein College of Medicine Bronx, NY 10461, USA

*To whom correspondence should be addressed

Received August 28, 1986. Accepted December 1, 1986.

Members of several classes of proto-oncogenes replicate during the first third of S-phase in two human (K562 erythroleukemia and HeLa), one Chinese hamster (CHO) and eight mouse cell lines. These cell lines exhibit a variety of specialized functions characteristic of pre-B and B cells, T cells and erythroid cells. The proto-oncogenes studied include fos, myc, myb, abl, fes, fms, mos, raf, rel, sis, Ha-ras, Ki-ras, and N-ras. In K562 cells, amplified and rearranged c-abl genes show a pattern of temporal replication during S that is similar to the pattern observed for the 5' breakpoint cluster region (bcr) and the amplified C{lambda} light chain immunoglobulin genes. The c-Ki-ras related sequences in CHO cells provide one example of late replicating proto-oncogene sequences that are present in multiple copies. The cellular gene N-myc replicates late during S in some of these cell lines. In three pre-B cell lines in which N-myc specific transcripts have been detected, N-myc replicates earlier in the S phase than in the other cell lines studied here.


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