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Nucleic Acids Research, 1987, Vol. 15, No. 10 4065-4084
© 1987

Articles

An unusually compact ribosomal DNA repeat in the protozoan Giardia lamblia

John C. Boothroyd1, Alice Wang2, David A. Campbell1,3 and Ching C. Wang2

1Department of Medical Microbiology, Stanford University School of Medicine Stanford, CA 94306 2Department of Pharmaceutical Chemistry, School of Pharmacy, University of California San Francisco, CA 94143 3Department of Microbiology and Immunology, School of Medicine, University of California Los Angeles, CA 90024, USA

Received January 12, 1987. Revised April 29, 1987. Accepted April 29, 1987.

The ribosomal RNA (rRNA) genes of the protozoan parasite Giardia lamblia have been analyzed with respect to size, composition and copy number. They are found to be remarkable in several respects. First, the rRNAs themselves are the smallest yet reported for any eukaryotic organism. Second, the genes encoding them are found as an exceptionally small tandemly repeated unit of only 5.4 kilobase-pairs. Third, the genes are extraordinarily G:C rich, even in regions which are highly conserved between all other eukaryotic rRNA genes. Finally, by analogy to other organisms, the 5.8S RNA appears to lack about 15 nucleotides from its 3'-end, a region previously thought to be essential for 5.8S RNA function. We also provide the first estimates of the genomic complexity and total G:C content of this important protozoan pathogen.


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