Nucleic Acids Research, 1987, Vol. 15, No. 10 4131-4143
© 1987
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Natural point mutations within rat rDNA transcription terminator elements reveal the functional importance of single bases for factor binding and termination
Institut für Biochemie Röntgenring 11, 8700 W
rzburg, FRG
Received March 13, 1987. Revised April 23, 1987. Accepted April 23, 1987.
The rat rDNA transcription unit extends 560–565 bp into the spacer down stream of the 28S rRNA coding region. The site of 3' end formation is located in front of a conserved 18 bp sequence element which is repeated eight times in the 3' spacer between nucleotides +582 and +1767 relative to the 3' terminus of 28S rRNA. These sequence motifs are almost identical to the RNA polymerase I transcription termination signal (the Sal I box) that has previously been identified in the 3' terminal spacer of mouse rDNA. Interestingly, each of the single rat elements contains one or more base substitutions as compared to the murine Sal I box. Individual rat Sal I boxes were cloned and tested for their ability to interact with the murine termination factor and to direct transcription termination. It is shown that five of the eight boxes represent genuine transcription terminators, while three elements contain certain point mutations which are not recognized by the nuclear Sal I box-binding protein and therefore are functionally inactive.
*Permanent address: Institute of Cell Biology and Morphology, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria
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